Over the recent years, the study of emotional functioning has become one of the central issues in dog cognition. Previous studies showed that dogs can recognize different emotions by looking at human faces and can correctly match the human emotional state with a vocalization having a negative emotional valence. However, to this day, little is known about how dogs perceive and process human non-verbal vocalizations having different emotional valence. The current research provides new insights into emotional functioning of the canine brain by studying dogs' lateralized auditory functions (to provide a first insight into the valence dimension) matched with both behavior and physiological measures of arousal (to study the arousal dimension) in response to playbacks related to the Ekman's six basic human emotions. Overall, our results indicate lateralized brain patterns for the processing of human emotional vocalizations, with the prevalent use of the right hemisphere in the analysis of vocalizations with a clear negative emotional valence (i.e. "fear" and "sadness") and the prevalent use of the left hemisphere in the analysis of positive vocalization ("happiness"). Furthermore, both cardiac activity and behavior response support the hypothesis that dogs are sensitive to emotional cues of human vocalizations.