The complexation of rhizosphere and nonrhizosphere soil organic matter with chromium: Using elemental analysis combined with FTIR spectroscopy

Complexation is a main mechanism controlling the reactions between soil organic matter (SOM) and heavy metals, which still have not been fully understood up to date. The objective of this study was to compare the SOM composition of nonrhizosphere and rhizosphere in low Cr treatment with that in high Cr treatment and to find out how metal concentrations affect the complexation with SOM. The results revealed that both the hydroxyl and the carboxyl were significantly different under different Cr treatment groups. For nonrhizosphere samples, the high Cr treatment tended to have less hydroxyl contents and more structural changes on hydroxyl (3389-3381 cm^-1) than the low Cr treatment (3389-3388 cm^-1), while in the rhizosphere samples the reverse happened. The gap of the different Cr treated band area in the rhizosphere samples (44 a.u of the gap) was greatly smaller than that in the nonrhizosphere samples (576 a.u of the gap). In both the rhizosphere and nonrhizosphere samples, the high Cr treatment showed greater structural changes on carboxylic acids (11, 12 a.u changes based on the control) than the low Cr treatment (4, 6 a.u). The unsaturated carboxylic acids could account for downward frequency shift and the contents in the nonrhizosphere samples were slightly greater than that in the rhizosphere samples. This study used elemental analysis combined with FTIR spectroscopy to explore the effects of metal concentrations on the complexation of Cr with SOM and the composition of SOM. These findings give a way to understanding part of the complexation mechanisms between the metal and SOM.