Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Kate E Keller; Sanjoy K Bhattacharya; Theresa Borrás; Thomas M Brunner; Sunee Chansangpetch; Abbott F Clark; W Michael Dismuke; Yiqin Du; Michael H Elliott; C Ross Ethier; Jennifer A Faralli; Thomas F Freddo; Rudolf Fuchshofer; Michael Giovingo; Haiyan Gong; Pedro Gonzalez; Alex Huang; Murray A Johnstone; Paul L Kaufman; Mary J Kelley; Paul A Knepper; Casey C Kopczynski; John G Kuchtey; Rachel W Kuchtey; Markus H Kuehn; Raquel L Lieberman; Shan C Lin; Paloma Liton; Yutao Liu; Elke Lütjen-Drecoll; Weiming Mao; Marisse Masis-Solano; Fiona McDonnell; Colleen M McDowell; Darryl R Overby; Padmanabhan P Pattabiraman; Vijay K Raghunathan; P Vasanth Rao; Douglas J Rhee; Uttio Roy Chowdhury; Paul Russell; John R Samples; Donald Schwartz; Evan B Stubbs; Ernst R Tamm; James C Tan; Carol B Toris; Karen Y Torrejon; Janice A Vranka; Mary K Wirtz; Thomas Yorio; Jie Zhang; Gulab S Zode; Michael P Fautsch; Donna M Peters; Ted S Acott; W Daniel Stamer

doi:10.1016/j.exer.2018.03.001

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Exp Eye Res. 2018 Jun:171:164-173. doi: 10.1016/j.exer.2018.03.001. Epub 2018 Mar 9.

Authors

Kate E Keller¹, Sanjoy K Bhattacharya², Theresa Borrás³, Thomas M Brunner⁴, Sunee Chansangpetch⁵, Abbott F Clark⁶, W Michael Dismuke⁷, Yiqin Du⁸, Michael H Elliott⁹, C Ross Ethier¹⁰, Jennifer A Faralli¹¹, Thomas F Freddo¹², Rudolf Fuchshofer¹³, Michael Giovingo¹⁴, Haiyan Gong¹⁵, Pedro Gonzalez¹⁶, Alex Huang¹⁷, Murray A Johnstone¹⁸, Paul L Kaufman¹¹, Mary J Kelley¹, Paul A Knepper¹⁹, Casey C Kopczynski⁷, John G Kuchtey²⁰, Rachel W Kuchtey²⁰, Markus H Kuehn²¹, Raquel L Lieberman¹⁰, Shan C Lin⁵, Paloma Liton¹⁶, Yutao Liu²², Elke Lütjen-Drecoll²³, Weiming Mao⁶, Marisse Masis-Solano⁵, Fiona McDonnell¹⁶, Colleen M McDowell⁶, Darryl R Overby²⁴, Padmanabhan P Pattabiraman²⁵, Vijay K Raghunathan²⁶, P Vasanth Rao¹⁶, Douglas J Rhee²⁵, Uttio Roy Chowdhury²⁷, Paul Russell²⁸, John R Samples²⁹, Donald Schwartz³⁰, Evan B Stubbs³¹, Ernst R Tamm¹³, James C Tan³², Carol B Toris²⁵, Karen Y Torrejon³³, Janice A Vranka¹, Mary K Wirtz¹, Thomas Yorio⁶, Jie Zhang¹⁷, Gulab S Zode⁶, Michael P Fautsch³⁴, Donna M Peters³⁵, Ted S Acott³⁶, W Daniel Stamer³⁷

Affiliations

¹ Oregon Health and Science University, United States.
² University of Miami, United States.
³ University of North Carolina-Chapel Hill, United States.
⁴ Glaucoma Research Foundation, United States.
⁵ University of California, San Francisco, United States.
⁶ University of North Texas Health Sciences Center, United States.
⁷ Aerie Pharmaceuticals, United States.
⁸ University of Pittsburgh, United States.
⁹ University of Oklahoma, United States.
¹⁰ Georgia Institute of Technology, United States.
¹¹ University of Wisconsin, United States.
¹² Massachusetts College of Pharmacy and Health Sciences, United States.
¹³ University of Regensburg, Germany.
¹⁴ John H. Stroger, Jr. Hospital of Cook County, United States.
¹⁵ Boston University, United States.
¹⁶ Duke University, United States.
¹⁷ University of California, Los Angeles, United States.
¹⁸ University of Washington, United States.
¹⁹ University of Illinois at Chicago, United States.
²⁰ Vanderbilt University, United States.
²¹ University of Iowa, United States.
²² Augusta University, United States.
²³ University of Erlangen-Nuremberg, Germany.
²⁴ Imperial College, London, United Kingdom.
²⁵ Case Western Reserve University, United States.
²⁶ University of Houston, United States.
²⁷ Mayo Clinic, United States.
²⁸ University of California, Davis, United States.
²⁹ Western Glaucoma Foundation, United States.
³⁰ EyeSonix, United States.
³¹ Loyola University, Chicago, United States.
³² University of Southern California, United States.
³³ Glauconix, United States.
³⁴ Department of Ophthalmology, Mayo Clinic, 200 1st St SW, Rochester, MN 55905, United States. Electronic address: [email protected].
³⁵ Department of Pathology & Laboratory Medicine, University of Wisconsin, 1300 University Ave, Madison, WI 53706, United States. Electronic address: [email protected].
³⁶ Department of Ophthalmology, Department of Biochemistry & Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, United States. Electronic address: [email protected].
³⁷ Department of Ophthalmology, Duke University, DUMC 3802, Durham, NC 27705, United States. Electronic address: [email protected].

Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

Keywords: Aqueous humor dynamics; Conventional outflow; Glaucoma; Intraocular pressure; Ocular hypertension.

Publication types

Review

MeSH terms

Age Factors
Animals
Biomarkers / metabolism
Cell Culture Techniques*
Cell Separation / methods*
Consensus
Fetus
Guidelines as Topic*
Humans
Tissue Donors
Tissue Preservation
Tissue and Organ Harvesting
Trabecular Meshwork / cytology*
Trabecular Meshwork / metabolism

Substances

Biomarkers

Abstract

Publication types

MeSH terms

Substances

Grants and funding