Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Sabitri Ghimire; Margot Van der Jeught; Jitesh Neupane; Matthias S Roost; Jasper Anckaert; Mina Popovic; Filip Van Nieuwerburgh; Pieter Mestdagh; Jo Vandesompele; Dieter Deforce; Björn Menten; Susana Chuva de Sousa Lopes; Petra De Sutter; Björn Heindryckx

doi:10.1038/s41598-018-24051-5

Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Sci Rep. 2018 Apr 12;8(1):5884. doi: 10.1038/s41598-018-24051-5.

Authors

Affiliations

¹ Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Corneel Heymanslaan 10, 9000, Ghent, Belgium. [email protected].
² Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
³ Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.
⁴ Department of Pediatrics and Medical Genetics, Ghent University Hospital, Corneel Heymanslaan 10, 9000, Gent, Belgium.
⁵ Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium.
⁶ Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Corneel Heymanslaan 10, 9000, Ghent, Belgium. [email protected].

Abstract

Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Embryo, Mammalian
Fibroblast Growth Factor 5 / genetics
Fibroblast Growth Factor 5 / metabolism
GATA6 Transcription Factor / genetics
GATA6 Transcription Factor / metabolism
Gene Expression Regulation, Developmental*
Gene Ontology
Genetic Background
HMGB Proteins / genetics
HMGB Proteins / metabolism
Keratin-18 / genetics
Keratin-18 / metabolism
Mice
Molecular Sequence Annotation
Mouse Embryonic Stem Cells / cytology
Mouse Embryonic Stem Cells / metabolism*
Nanog Homeobox Protein / genetics
Nanog Homeobox Protein / metabolism
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / metabolism*
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism
SOXF Transcription Factors / genetics
SOXF Transcription Factors / metabolism
Signal Transduction
Transcriptome*

Substances

Fgf5 protein, mouse
GATA6 Transcription Factor
Gata6 protein, mouse
HMGB Proteins
Keratin-18
Nanog Homeobox Protein
Nanog protein, mouse
Octamer Transcription Factor-3
Pou5f1 protein, mouse
SOXB1 Transcription Factors
SOXF Transcription Factors
Sox17 protein, mouse
Sox2 protein, mouse
Fibroblast Growth Factor 5