Crystallization of and preliminary crystallographic data for Bacillus stearothermophilus cyclodextrin glucanotransferase

J Biochem. 1988 Jul;104(1):12-3. doi: 10.1093/oxfordjournals.jbchem.a122406.

Abstract

Cyclodextrin glucanotransferase from Bacillus stearothermophilus TC-91 has been crystallized from an ammonium sulfate solution by the dialysis equilibrium method. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with cell dimensions of a = 125.5 A, b = 88.1 A, and c = 81.5 A. The crystals appear to be suitable for X-ray structure analysis, diffracting to at least 2.1 A and being resistant to radiation damage.

MeSH terms

  • Chromatography, Ion Exchange
  • Crystallization
  • Geobacillus stearothermophilus / enzymology*
  • Glucosyltransferases* / isolation & purification
  • Protein Conformation
  • Ultrafiltration
  • X-Ray Diffraction

Substances

  • Glucosyltransferases
  • cyclomaltodextrin glucanotransferase