Consensus guidelines for the use and interpretation of angiogenesis assays

Affiliations

• ¹ Molecular Pharmacology Group, School of Pharmaceutical Sciences, Faculty of Sciences, University of Geneva, University of Lausanne, Rue Michel-Servet 1, CMU, 1211, Geneva 4, Switzerland. [email protected].
• ² Translational Research Center in Oncohaematology, University of Geneva, Geneva, Switzerland. [email protected].
• ³ Wihuri Research Institute and Translational Cancer Biology Program, University of Helsinki, Helsinki, Finland.
• ⁴ Laboratory of Tumor Microenvironment and Therapeutic Resistance, Department of Oncology, VIB-Center for Cancer Biology, KU Leuven, Louvain, Belgium.
• ⁵ Department of Pathology, University of Washington, Seattle, WA, USA.
• ⁶ University of Wisconsin, Madison, WI, USA.
• ⁷ European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany.
• ⁸ Division of Vascular Oncology and Metastasis Research, German Cancer Research Center, Heidelberg, Germany.
• ⁹ German Cancer Consortium, Heidelberg, Germany.
• ¹⁰ Division of Cancer and Stem Cells, School of Medicine, University of Nottingham, Nottingham, UK.
• ¹¹ Angiogenesis Laboratory, Department of Medical Oncology, VU University Medical Center, Cancer Center Amsterdam, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
• ¹² Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA.
• ¹³ Department of Neurological Surgery, Brain Tumor Research Center, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
• ¹⁴ Angiogenesis and Tumor Microenvironment Laboratory (INSERM U1029), University Bordeaux, Pessac, France.
• ¹⁵ Vascular Biology Program and Department of Surgery, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
• ¹⁶ Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME, USA.
• ¹⁷ Department of Oncology, University of Torino, Turin, Italy.
• ¹⁸ Candiolo Cancer Institute-FPO-IRCCS, 10060, Candiolo, Italy.
• ¹⁹ Department of Ophthalmology, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
• ²⁰ Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.
• ²¹ Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Leuven, Belgium.
• ²² Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
• ²³ Department of Microscopic Morphology/Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
• ²⁴ Department of Molecular Biology, Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA.
• ²⁵ Ludwig Institute for Cancer Research, Department of Oncology, University of Lausanne, Lausanne, Switzerland.
• ²⁶ Department of Medical Pharmacology and Physiology, University of Missouri, School of Medicine and Dalton Cardiovascular Center, Columbia, MO, USA.
• ²⁷ School of Life Sciences, Swiss Federal Institute of Technology, Lausanne, Switzerland.
• ²⁸ Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
• ²⁹ Department of Radiation Oncology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
• ³⁰ Institute of Anatomy, University of Bern, Bern, Switzerland.
• ³¹ Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.
• ³² Emily Couric Cancer Center, The University of Virginia, Charlottesville, VA, USA.
• ³³ Vascular Sciences, Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, London, UK.
• ³⁴ Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, Leuven, Belgium.
• ³⁵ Laboratory of Cellular Metabolism and Metabolic Regulation, Department of Oncology, KU Leuven and Leuven Cancer Institute, Leuven, Belgium.
• ³⁶ University of California, San Diego, La Jolla, CA, USA.
• ³⁷ Institute of Ophthalmology, University College London, London, UK.
• ³⁸ Edwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
• ³⁹ Metabolomics Expertise Center, VIB Center for Cancer Biology, VIB, Leuven, Belgium.
• ⁴⁰ Department of Oncology, Metabolomics Expertise Center, KU Leuven, Leuven, Belgium.
• ⁴¹ Molecular Oncology Laboratories, Oxford University Department of Oncology, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
• ⁴² MCDB, University of California, Los Angeles, CA, USA.
• ⁴³ Department of Cancer Biology, Metastasis Research Center, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
• ⁴⁴ Department of Medical Biophysics, Biological Sciences Platform, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.
• ⁴⁵ Department of Physiology and Biophysics, University of Illinois, Chicago, IL, USA.
• ⁴⁶ Ocular Angiogenesis Group, Departments of Ophthalmology and Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
• ⁴⁷ The George Washington University School of Medicine, Washington, DC, USA.
• ⁴⁸ Department of Ophthalmology, University of Lausanne, Jules-Gonin Eye Hospital, Fondation Asile des Aveugles, Lausanne, Switzerland.
• ⁴⁹ Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
• ⁵⁰ HistoGeneX, Antwerp, Belgium.
• ⁵¹ Department of Cardiac Surgery, Harvard Medical School, Boston Children's Hospital, Boston, MA, USA.
• ⁵² Pathology and Laboratory Medicine Service, VA Puget Sound Health Care System, Seattle, WA, USA.
• ⁵³ Laboratory of Tumor and Developmental Biology, GIGA-Cancer, University of Liège, Liège, Belgium.
• ⁵⁴ Department of Biotechnology and Molecular Medicine, University of Eastern Finland, Kuopio, Finland.
• ⁵⁵ Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden.
• ⁵⁶ Department of oncology UNIL-CHUV, Ludwig Institute for Cancer Research Lausanne, Lausanne, Switzerland.
• ⁵⁷ Division of Translational Cancer Research, Department of Laboratory Medicine, Lund, Sweden.
• ⁵⁸ Genitourinary Program, Indiana University-Simon Cancer Center, Indianapolis, IN, USA.
• ⁵⁹ Medical Research Council Centre for Reproductive Health, College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, UK.
• ⁶⁰ Department of Physiology, Maastricht University, Maastricht, The Netherlands.
• ⁶¹ Einthoven Laboratory for Experimental Vascular Medicine, Department Surgery, LUMC, Leiden, The Netherlands.
• ⁶² Laboratory of Immunopathology, Institute of Biology and Experimental Medicine, National Council of Scientific and Technical Investigations (CONICET), Buenos Aires, Argentina.
• ⁶³ Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari Medical School, Bari, Italy.
• ⁶⁴ National Cancer Institute "Giovanni Paolo II", Bari, Italy.
• ⁶⁵ Department of Oncology, Microbiology and Immunology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
• ⁶⁶ Institute of Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, WWU, Münster, Germany.
• ⁶⁷ Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, USA.
• ⁶⁸ Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
• ⁶⁹ Tumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre and The Sir Peter MacCallum, Department of Oncology, University of Melbourne, Melbourne, VIC, Australia.
• ⁷⁰ Translational Cancer Research Unit, GZA Hospitals, Sint-Augustinus & University of Antwerp, Antwerp, Belgium.
• ⁷¹ Medical Faculty, University of Münster, Albert-Schweitzer-Campus 1, Münster, Germany.
• ⁷² Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA.
• ⁷³ Angiogenesis Laboratory, Department of Medical Oncology, VU University Medical Center, Cancer Center Amsterdam, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands. [email protected].