Isoprostanes as markers for muscle aging in older athletes

Claire Vinel; Ophélie Pereira; Aude Dupuy; Justine Bertrand-Michel; Dalila Laoudj-Chenivesse; Yves Rolland; Daniel Rivière; Philippe Valet; Cédric Dray; Fabien Pillard

doi:10.1016/j.biopen.2017.12.001

Isoprostanes as markers for muscle aging in older athletes

Biochim Open. 2017 Dec 15:6:1-8. doi: 10.1016/j.biopen.2017.12.001. eCollection 2018 Jun.

Authors

Claire Vinel¹, Ophélie Pereira², Aude Dupuy³, Justine Bertrand-Michel³, Dalila Laoudj-Chenivesse⁴, Yves Rolland^{5

6}, Daniel Rivière^{1

2

7}, Philippe Valet¹, Cédric Dray¹, Fabien Pillard^{1

2

7}

Affiliations

¹ Institute of Metabolic and Cardiovascular Diseases, Joint Research Unit 1048 INSERM Adipolab Unit - Paul Sabatier University, Toulouse, France.
² Exercise Physiology Department, Medical School, Paul Sabatier University, Toulouse, France.
³ MetaToul Lipidomic Core Facility, MetaboHUB, Joint Research Unit 1048 INSERM - Paul Sabatier University, Toulouse, France.
⁴ Experimental Heart and Muscle Physiology and Medicine, Joint Research Unit 1046 INSERM - University of Montpellier 1 and 2, Montpellier, France.
⁵ Gérontopôle of Toulouse, Institute of Aging, University Hospital, Toulouse, France.
⁶ Epidemiology and Chronic Disease, Joint Research Unit 1027 INSERM - Paul Sabatier University, Toulouse, France.
⁷ Sport Medicine Department, Larrey University Hospital, Toulouse, France.

Abstract

Introduction: Production of isoprostanes (IsoPs) is enhanced after acute, intense, and prolonged exercise, in untrained subjects. This effect is greater in older subjects. The present study aims to delineate the profile of acute-exercise-induced IsoPs levels in young and older endurance-trained subjects.

Methods: All included subjects were male, young (n = 6; 29 yrs ± 5.7) or older (n = 6; 63.7 yrs ± 2.3), and competitors. The kinetics of F₂-IsoPs in blood-sera was assessed at rest, for the maximal aerobic exercise power (MAP) corresponding to the cardio-respiratory fitness index and after a 30-min recovery period.

Results: No significant time effect on F₂-IsoPs kinetics was identified in young subjects. However, in older athletes, F₂-IsoPs blood-concentrations at the MAP were higher than at rest, whereas these blood-concentrations did not differ between rest and after the 30-min recovery period.

Conclusion: Because plasma glutathione (GSH) promotes the formation of some F₂-IsoPs, we suggest that the surprising decrease in F₂-IsoPs levels in older subjects would be caused by decreased GSH under major ROS production in older subjects. We argue that the assessment F₂-IsoPs in plasma as biomarkers of the aging process should be challenged by exercise to improve the assessment of the functional response against reactive oxygen species in older subjects.

Keywords: $\dot{V} O_{2} max$ , Maximal oxygen uptake; Aging; BHT, Butylated hydroxytoluene; Exercise; FSHD, Facioscapulohumeral dystrophy; GSH, Glutathione; HPLC, High-performance liquid chromatography; IsoP, Isoprostane; Isoprostanes; La30, Venous blood-lactate concentration at 30 min after exercise; Lamax, Venous blood-lactate concentration at $\dot{V} O_{2} max$ ; MAP, Maximal aerobic power; MS, Mass spectrometry; Nrf2, Erythroid 2-like factor 2; ROS, reactive-oxygen species; Training.