[Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma]

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Jan 8;33(1):78-82. doi: 10.12047/j.cjap.5448.2017.020.
[Article in Chinese]

Abstract

Objective: To investigate the effects of long non-coding RNA SPRY4-IT1 (LncRNA) on proliferation and metastasis of medul-loblastoma cells.

Methods: SPRY4-IT1siRNA and control fluorescence siRNA were transfected into medulloblastoma cell line Daoy with Lipo-fe ctamine 2000 and were divided into control group and si-SPRY4-IT1 group. Relative expression of SPRY4-IT1 mRNA were detected by real-time quantitative PCR. The change of cell proliferation were examined using CCK-8 kit and clone forming experiment. The change of cell inva-sion and metastasis were examined by matrigel invasion assay and cell metastasis experiment respectively, The expression of matrix metallopro-teinase MMP-2 and MMP-9 were detected by Western blot.

Results: In si-SPRY4-IT1 group,the SPRY4-IT1 mRNA expression level, cell pro-liferation in vitro,cell invasion and migration ability, MMP-2 protein expression were significantly lower than those in the control group.

Conclusions: Interference SPRY4-IT1 expression has prominent inhibitory effect on the cell proliferation、invasion and metastasis of medulloblastoma cell line Daoy.

Keywords: SPRY4-IT1; invasion and metastasis; long noncoding RNA (LncRNA); medulloblastoma; proliferation.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation*
  • Cerebellar Neoplasms / pathology*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Matrix Metalloproteinase 2 / metabolism
  • Matrix Metalloproteinase 9 / metabolism
  • Medulloblastoma / pathology*
  • Neoplasm Metastasis*
  • RNA, Long Noncoding / genetics*

Substances

  • RNA, Long Noncoding
  • long noncoding RNA SPRY4-IT1, human
  • MMP2 protein, human
  • Matrix Metalloproteinase 2
  • MMP9 protein, human
  • Matrix Metalloproteinase 9