Abstract
Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alveolar Epithelial Cells / cytology
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Alveolar Epithelial Cells / physiology*
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Cell Adhesion
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Cell Culture Techniques / methods*
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Cell Movement
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Cells, Cultured
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Coculture Techniques
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Dendritic Cells / cytology
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Dendritic Cells / physiology*
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Humans
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Image Processing, Computer-Assisted
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Imaging, Three-Dimensional / methods*
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Macrophages / cytology
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Macrophages / physiology*
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Microscopy, Fluorescence
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Nanoparticles / chemistry
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Time-Lapse Imaging / methods*
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Wound Healing