Analysis of the structures of the subunits of the cytochrome bc1 complex from beef heart mitochondria

FEBS Lett. 1986 Aug 11;204(1):9-15. doi: 10.1016/0014-5793(86)81378-3.

Abstract

The interaction of the protein subunits of the bc1 complex from beef heart is analysed on the basis of protein chemical data and of secondary structure predictions suggesting a large number of amphipathic helices. Electrostatic interactions, i.e. helix-dipole interactions and ionic bonds, may play a major role in the stabilisation of the arrangement of the subunits within the multi-protein complex, formation of subcomplexes and maintenance of the steric strain of cytochrome b. A model of the heme-carrying 'core' of cytochrome b, i.e. of helices II-V, is presented consisting of a twisted '4-alpha-helical' bundle held together by helix-dipole interactions and stabilised by the interaction with other protein subunits of the bc1 complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Electron Spin Resonance Spectroscopy
  • Electron Transport Complex III
  • Heme / metabolism
  • Macromolecular Substances
  • Mitochondria, Heart / enzymology*
  • Molecular Conformation
  • Multienzyme Complexes / analysis*
  • Oxidation-Reduction
  • Protein Conformation
  • Quinone Reductases / analysis*

Substances

  • Macromolecular Substances
  • Multienzyme Complexes
  • Heme
  • Quinone Reductases
  • Electron Transport Complex III