Computational Simulation of Array-based Electroporation in the Cochlea

Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul:2018:2462-2465. doi: 10.1109/EMBC.2018.8512808.

Abstract

We present a computational model for predicting electric field distributions following array-based closed-loop electroporation in the cochlea. The model geometry was reconstructed from magnetic resonance images of the guinea pig cochlea and an eight-channel electrode array embedded within this geometry. The model's electrode voltage output waveform was obtained from electric potential mapping conducted in physiological solution following constant-current stimulation using the electrode array. Our simulations predict that a tandem electrode configuration with four ganged cathodes and four ganged anodes produces a larger area in target tissue where the electric field is within the range for successful gene transfer compared to an alternate paired anode-cathode electrode configuration. These findings corroborate published in vivo evidence comparing the two configurations and support the utility of the developed model as a tool to optimize the efficacy of electroporation electrodes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cochlea
  • Electrodes
  • Electroporation*
  • Genetic Therapy
  • Guinea Pigs