We have developed a mid-infrared (mid-IR) tunable Cr:CdSe laser with nanosecond pulse operation. A broad tuning range from 2.25 to 3.08 µm and an output energy exceeding 4 mJ at 2.64 μm were demonstrated. The maximum energy conversion for absorbed energy reached 35% when the pump fluence was 2.1 J/cm2. We showed that Cr:CdSe is an attractive laser material for obtaining high-energy pulses in the mid-IR region and that the Cr:CdSe laser has high potential for laser biomaterial processing.