Sequential cultivation of microalgae in raw and recycled dairy wastewater: Microalgal growth, wastewater treatment and biochemical composition

Ehsan Daneshvar; Mohammad Javad Zarrinmehr; Eleni Koutra; Michael Kornaros; Omidvar Farhadian; Amit Bhatnagar

doi:10.1016/j.biortech.2018.11.059

Sequential cultivation of microalgae in raw and recycled dairy wastewater: Microalgal growth, wastewater treatment and biochemical composition

Bioresour Technol. 2019 Feb:273:556-564. doi: 10.1016/j.biortech.2018.11.059. Epub 2018 Nov 16.

Authors

Ehsan Daneshvar¹, Mohammad Javad Zarrinmehr², Eleni Koutra³, Michael Kornaros⁴, Omidvar Farhadian⁵, Amit Bhatnagar⁶

Affiliations

¹ Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland. Electronic address: [email protected].
² Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland; Department of Natural Resources, Isfahan University of Technology, Isfahan 8415683111, Iran.
³ Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, 26504 Patras, Greece.
⁴ Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, 26504 Patras, Greece; INVALOR: Research Infrastructure for Waste Valorization and Sustainable Management, 1 Karatheodori Str., University Campus, 26504 Patras, Greece.
⁵ Department of Natural Resources, Isfahan University of Technology, Isfahan 8415683111, Iran.
⁶ Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.

PMID: 30476864
DOI: 10.1016/j.biortech.2018.11.059

Abstract

In this study, two cycles of mixotrophic and one cycle of heterotrophic cultivation of Scenedesmus quadricauda (freshwater) and Tetraselmis suecica (marine water) microalgae in dairy wastewater (DWW) were investigated. Dry weights of S. quadricauda and T. suecica were found to be 0.43 and 0.58 g/L after the first cycle and 0.36, and 0.65 g/L after the second cycle of mixotrophic cultivation, respectively. Chlorophyll a content of both microalgae in the first cycle was significantly higher than the second cycle. S. quadricauda removed 92.15% of total nitrogen, 100% of phosphate, 100% of sulfate and 76.77% of total organic carbon, after two cycles of cultivation. The dominant fatty acids during the first and second cycle of S. quadricauda and T. suecica cultivation were C18:1 and C18:3n-3, respectively. The results suggest that by reusing DWW in two consecutive cycles of microalgal cultivation, higher pollutants removal efficiency and microalgal biomass production can be achieved.

Keywords: Dairy wastewater (DWW); Fatty acid methyl esters (FAMEs); Microalgae; Mixotrophic cultivation; Wastewater reuse; Wastewater treatment.

MeSH terms

Biomass
Chlorophyll A / metabolism
Fatty Acids / metabolism
Fresh Water
Microalgae / metabolism*
Nitrogen / metabolism
Recycling
Scenedesmus / metabolism*
Wastewater / chemistry*

Substances

Fatty Acids
Waste Water
Nitrogen
Chlorophyll A