FUT3 and FUT2 genotyping and glycoconjugate profile Lewisb as a protective factor to Toxoplasma gondii infection

Fabiana Nakashima; Cinara Cássia Brandão de Mattos; Ana Iara Costa Ferreira; Lígia Cosentino Junqueira Franco Spergiorin; Cristina Silva Meira-Strejevitch; Antonio Hélio Oliani; Denise Cristina Mós Vaz-Oliani; Vera Lúcia Pereira-Chioccola; Luiz Carlos de Mattos

doi:10.1016/j.actatropica.2019.02.031

FUT3 and FUT2 genotyping and glycoconjugate profile Lewis^b as a protective factor to Toxoplasma gondii infection

Acta Trop. 2019 May:193:92-98. doi: 10.1016/j.actatropica.2019.02.031. Epub 2019 Mar 1.

Authors

Fabiana Nakashima¹, Cinara Cássia Brandão de Mattos², Ana Iara Costa Ferreira³, Lígia Cosentino Junqueira Franco Spergiorin⁴, Cristina Silva Meira-Strejevitch⁵, Antonio Hélio Oliani⁶, Denise Cristina Mós Vaz-Oliani⁷, Vera Lúcia Pereira-Chioccola⁸, Luiz Carlos de Mattos⁹

Affiliations

¹ Biology Department, Bioscience, Languages and Exact Sciences Institute of the Universidade Estadual Paulista "Júlio de Mesquita Filho" (IBILCE/UNESP), São José do Rio Preto, São Paulo, Brazil; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil. Electronic address: [email protected].
² Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil; FAMERP Toxoplasma Research Group, Brazil. Electronic address: [email protected].
³ Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil. Electronic address: [email protected].
⁴ Gynecology and Obstetrics Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil; FAMERP Toxoplasma Research Group, Brazil. Electronic address: [email protected].
⁵ Parasite Molecular Biology Laboratory, Instituto Adolfo Lutz, São Paulo, Brazil. Electronic address: [email protected].
⁶ Gynecology and Obstetrics Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil. Electronic address: [email protected].
⁷ Gynecology and Obstetrics Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil. Electronic address: [email protected].
⁸ Parasite Molecular Biology Laboratory, Instituto Adolfo Lutz, São Paulo, Brazil. Electronic address: [email protected].
⁹ Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil; FAMERP Toxoplasma Research Group, Brazil. Electronic address: [email protected].

PMID: 30831115
DOI: 10.1016/j.actatropica.2019.02.031

Abstract

The interaction between the ABO, FUT2 and FUT3 genes results in the synthesis of different glycoconjugates profiles expressed in gastrointestinal tract. Moreover, the protozoan Toxoplasma gondii, which causes toxoplasmosis, utilizes this organ as an infection route. We analyzed the frequencies of the different glycoconjugate profiles which were determined by phenotyping ABO and genotyping the status secretor (FUT2; substitution G428A) and Lewis (FUT3; substitution T202C and C314T) histo-blood systems, assessed by PCR-RFLP and PCR-SSP, respectively. A total of 244 pregnant women (G1: Seropositive; G2: Seronegative) for IgG T. gondii antibodies were enrolled. IgG anti-T. gondii antibodies were determined by ELISA. G1 was composed of 158 (64.8%) sample and G2 by 86 (36.2%). The glycoconjugate profile was accessed in 151 seropositive and 85 seronegative samples by the combination of ABO and Lewis phenotyping as well as FUT2 and FUT3 genotyping. In G1, 36 (22.8%) presented the glycoconjugate profile ALe^b, 5 (3.3%) A, 13 (8.6) BLe^b, 1 (0.6%) B, 41 (27.1%) Le^b, 13(8.6%) H, 38(25.2%) Le^a and 4 (2.6%) Le^c. G2 was composed of 13 (15.3%) of ALe^b, 15 (17.6%) BLe^b, 1 (1.2%) B, 42 (49,4%) Le^b and 14 (16.5) Le^a. H and Le^c glycoconjugate profiles were not found in G2. The frequencies of the glycoconjugates profiles Le^b (p = 0.001) and H (p = 0.005) were significantly different compared between G1 and G2. The glycoconjugate profile H inferred from the ABO phenotyping and FUT3 and FUT2 genotyping is associated with infection by T. gondii in pregnant women and the Le^b profile appears to protect the infection by this parasite.

Keywords: Blood groups genotying; FUT2 gene; FUT3 gene; Lewis blood group system; Toxoplasma gondii; Toxoplasmosis.

MeSH terms

ABO Blood-Group System / blood
Adult
Antibodies, Protozoan / blood
Female
Fucosyltransferases / genetics*
Galactoside 2-alpha-L-fucosyltransferase
Genotype
Glycoconjugates / blood*
Humans
Immunoglobulin G / blood
Lewis Blood Group Antigens / blood
Pregnancy
Protective Factors
Toxoplasma / immunology
Toxoplasmosis / genetics*
Young Adult

Substances

ABO Blood-Group System
Antibodies, Protozoan
Glycoconjugates
Immunoglobulin G
Lewis Blood Group Antigens
Fucosyltransferases
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase