Background: Deoxynivalenol (DON) is a mycotoxin produced mainly by Fusarium graminearum. This fungus is the main plant pathogen associated with Fusarium Head Blight (FHB) wheat disease, causing significant economic losses and exposing human population to severe health risks. DON production changes widely among different years and areas and its effects are larger in years with abundant rainfall and high relative humidity. To date, Argentina has not established DON tolerance limits. Objective: To validate a method using GC with electron capture detector (GC-ECD) and to provide evidence of DON contamination in Argentinean commercial wheat flour. Results: A total of 34 different flour samples were analyzed obtaining 91.2% of incidence with a mean level of 243 µg/kg and a median level of 165 µg/kg. The method showed acceptable LOD (24 µg/kg) and LOQ (79 µg/kg), relative SD (RSD) of the intermediate precision (RSD = 5.98%), recovery (89.3%) and uncertainty (14%). Conclusions: The method was successfully validated according to the studied parameters. Incidence results for DON contamination are low and in accordance with previous studies for years with low FHB incidence in wheat.