Isolation, characterization, cloning and bioinformatics analysis of a novel receptor from black cut worm (Agrotis ipsilon) of Bacillus thuringiensis vip 3Aa toxins

Gamal H Osman; Raya Soltane; Ibrahim Saleh; Hussein H Abulreesh; Khaled S Gazi; Ibrahim A Arif; Ahmed M Ramadan; Hussien F Alameldin; Yehia A Osman; Mamdouh Idriss

doi:10.1016/j.sjbs.2018.06.002

Isolation, characterization, cloning and bioinformatics analysis of a novel receptor from black cut worm (Agrotis ipsilon) of Bacillus thuringiensis vip 3Aa toxins

Saudi J Biol Sci. 2019 Jul;26(5):1078-1083. doi: 10.1016/j.sjbs.2018.06.002. Epub 2018 Jun 13.

Authors

Gamal H Osman^{1

2}, Raya Soltane^{3

4}, Ibrahim Saleh⁵, Hussein H Abulreesh¹, Khaled S Gazi⁶, Ibrahim A Arif⁵, Ahmed M Ramadan^{7

8}, Hussien F Alameldin^{9

10}, Yehia A Osman¹¹, Mamdouh Idriss¹²

Affiliations

¹ Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makka, Saudi Arabia.
² Microbial Genetics Department, Agricultural Genetic Engineering Research Institute (AGERI), Giza, Egypt.
³ Faculty of Sciences of Tunis, Tunis El Manar University, Tunisia.
⁴ Department of basic sciences, Adham University college, Umm Al-Qura University, Saudi Arabia.
⁵ Prince Sultan Research Chair for Environment and Wildlife, Department of Botany & Microbiology, College of Sciences, King Saud University (KSU), Riyadh, Saudi Arabia.
⁶ Department of Biology, Faculty of Arts and Sciences in Almandaq, Albaha University, Saudi Arabia.
⁷ Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah 80203, Saudi Arabia.
⁸ Plant Molecular Biology Department, Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt.
⁹ Department of Energy - Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
¹⁰ Department of Bioinformatics, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt.
¹¹ Microbiology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
¹² Department of Entomology, Faculty of Agriculture, Alexandria University, Egypt.

Abstract

Black cutworm (BCW) is an economically important lepidopteran insect. The control of this insect by a Bt toxin and the understanding of the interaction between the Bt toxin and its receptor molecule were the objectives of this research work. A gene coding for a Vip3A receptor molecule was identified, characterized, and cloned, from the brush border membrane vesicles (BBMV) of the BCW. The nucleotide sequence analysis of the cloned putative Vip3A-receptor gene revealed that the gene was 1.3-kb long and exhibited no homology with any gene in the gene bank. We succeeded in identifying and characterizing most of the Vip3A-receptor gene sequence; and the nucleotide sequence analysis of the cloned putative Vip3A-receptor gene (accession no. KX858809) revealed about 92% of the expected sequence was recovered, which exhibited no homology with any gene in the GenBank.

Keywords: BBMV; Bacillus thuringiensis; Receptor; Vip3A.