Profile of a Highly Selective Quaternized Pyrrolidine Betaine αvβ6 Integrin Inhibitor-(3 S)-3-(3-(3,5-Dimethyl-1 H-pyrazol-1-yl)phenyl)-4-((1 S and 1 R,3 R)-1-methyl-3-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)pyrrolidin-1-ium-1-yl)butanoate Synthesized by Stereoselective Methylation

J Med Chem. 2019 Aug 22;62(16):7543-7556. doi: 10.1021/acs.jmedchem.9b00819. Epub 2019 Aug 5.

Abstract

A quaternary ammonium betaine 7 is described which shows exceptional potency and selectivity (1.4 to >3 logs) for the αvβ6 integrin receptor over the other αv integrins as determined in cell adhesion assays. 7 is prepared by remarkably stereoselective methylation, the origins of which are discussed. The chemical, biological, physicochemical, and pharmacokinetic properties of 7 and its docking into αvβ6 are described along with related analogues.

MeSH terms

  • Animals
  • Antigens, Neoplasm / chemistry
  • Antigens, Neoplasm / metabolism
  • Betaine / chemistry
  • Betaine / pharmacokinetics
  • Betaine / pharmacology*
  • Cells, Cultured
  • Crystallography, X-Ray
  • Hepatocytes / cytology
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Humans
  • Integrins / antagonists & inhibitors*
  • Integrins / chemistry
  • Integrins / metabolism
  • Methylation
  • Models, Chemical
  • Molecular Docking Simulation
  • Molecular Structure
  • Protein Binding
  • Protein Conformation
  • Pyrrolidines / chemistry*
  • Quaternary Ammonium Compounds / chemistry
  • Quaternary Ammonium Compounds / pharmacokinetics
  • Quaternary Ammonium Compounds / pharmacology*
  • Rats
  • Stereoisomerism

Substances

  • Antigens, Neoplasm
  • Integrins
  • Pyrrolidines
  • Quaternary Ammonium Compounds
  • integrin alphavbeta6
  • Betaine
  • pyrrolidine