Follicular Helper T Cells Are Major Human Immunodeficiency Virus-2 Reservoirs and Support Productive Infection

J Infect Dis. 2020 Jan 1;221(1):122-126. doi: 10.1093/infdis/jiz431.

Abstract

Follicular helper T cells (Tfh), CD4 lymphocytes critical for efficient antibody responses, have been shown to be key human immunodeficiency virus (HIV)-1 reservoirs. Human immunodeficiency virus-2 infection represents a unique naturally occurring model for investigating Tfh role in HIV/acquired immune deficiency syndrome, given its slow rate of CD4 decline, low to undetectable viremia, and high neutralizing antibody titers throughout the disease course. In this study, we investigated, for the first time, Tfh susceptibility to HIV-2 infection by combining in vitro infection of tonsillar Tfh with the ex vivo study of circulating Tfh from HIV-2-infected patients. We reveal that Tfh support productive HIV-2 infection and are preferential viral targets in HIV-2-infected individuals.

Keywords: HIV-2; HIV/AIDS; Tfh; follicular helper T cells; viral reservoirs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Viral / metabolism*
  • Female
  • HIV Infections / immunology*
  • HIV Infections / virology
  • HIV-1 / physiology*
  • HIV-2 / physiology*
  • Humans
  • Middle Aged
  • Palatine Tonsil / immunology
  • Palatine Tonsil / pathology
  • Primary Cell Culture
  • RNA, Messenger / metabolism
  • Receptors, CCR5 / metabolism
  • Receptors, CXCR4 / metabolism
  • T-Lymphocytes, Helper-Inducer / metabolism
  • T-Lymphocytes, Helper-Inducer / virology*
  • Viral Tropism
  • gag Gene Products, Human Immunodeficiency Virus / genetics

Substances

  • CCR5 protein, human
  • CXCR4 protein, human
  • DNA, Viral
  • RNA, Messenger
  • Receptors, CCR5
  • Receptors, CXCR4
  • gag Gene Products, Human Immunodeficiency Virus