Amino and PEG-amino graphene oxide grids enrich and protect samples for high-resolution single particle cryo-electron microscopy

J Struct Biol. 2020 Feb 1;209(2):107437. doi: 10.1016/j.jsb.2019.107437. Epub 2019 Dec 19.

Abstract

Cryo-EM samples prepared using traditional methods often suffer from too few particles, poor particle distribution, strongly biased orientation, or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and orientation. By introducing a PEG spacer, particles are kept away from both the GO surface and the air-water interface, protecting them from potential denaturation.

Keywords: Air-water interface; Amino functionalization; Cryo-EM; Graphene oxide; Sample preparation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / chemistry
  • Cryoelectron Microscopy / methods*
  • Graphite / chemistry*
  • Polyethylene Glycols / chemistry
  • Single Molecule Imaging / methods*
  • Water / chemistry*

Substances

  • Amines
  • graphene oxide
  • Water
  • Polyethylene Glycols
  • Graphite