Performance of a novel Next Generation Sequencing circulating tumor DNA (ctDNA) platform for the evaluation of samples from patients with metastatic breast cancer (MBC)

Crit Rev Oncol Hematol. 2020 Jan:145:102856. doi: 10.1016/j.critrevonc.2019.102856. Epub 2019 Dec 23.

Abstract

Circulating tumor DNA (ctDNA) is gaining momentum as sensitive diagnostic tool for advanced disease characterization because of its ability both to capture the tumor's heterogeneity and its dynamic adaptations. However, the consistency between all the available platforms is still debated. The aim of the study was to explore the performance of the novel diagnostic NGS platform PredicinePLUS™ and to compare its results with the clinically available Guardant360™ platform for possible analytical inconsistencies. The study suggests that PredicinePLUS™ NGS platform can detect genomic alterations and measure allele frequency in samples of MBC patients and confirmed that different NGS platforms could be potentially compared provided that certain sample management and analytical requirements are met.

Keywords: Circulating tumor DNA; Clinical utility; Next generation sequencing; Precision medicine.

Publication types

  • Review

MeSH terms

  • Biomarkers, Tumor*
  • Breast Neoplasms* / diagnosis
  • Breast Neoplasms* / genetics
  • Circulating Tumor DNA*
  • Genomics
  • High-Throughput Nucleotide Sequencing*
  • Humans
  • Mutation

Substances

  • Biomarkers, Tumor
  • Circulating Tumor DNA