Soybean adaption to high-latitude regions is associated with natural variations of GmFT2b, an ortholog of FLOWERING LOCUS T

Plant Cell Environ. 2020 Apr;43(4):934-944. doi: 10.1111/pce.13695. Epub 2020 Jan 25.

Abstract

Day length has an important influence on flowering and growth habit in many plant species. In crops such as soybean, photoperiod sensitivity determines the geographical range over which a given cultivar can grow and flower. The soybean genome contains ~10 genes homologous to FT, a central regulator of flowering from Arabidopsis thaliana. However, the precise roles of these soybean FTs are not clearly. Here we show that one such gene, GmFT2b, promotes flowering under long-days (LDs). Overexpression of GmFT2b upregulates expression of flowering-related genes which are important in regulating flowering time. We propose a 'weight' model for soybean flowering under short-day (SD) and LD conditions. Furthermore, we examine GmFT2b sequences in 195 soybean cultivars, as well as flowering phenotypes, geographical distributions and maturity groups. We found that Hap3, a major GmFT2b haplotype, is associated with significantly earlier flowering at higher latitudes. We anticipate our assay to provide important resources for the genetic improvement of soybean, including new germplasm for soybean breeding, and also increase our understanding of functional diversity in the soybean FT gene family.

Keywords: Glycine max (L.) Merr.; GmFT2b; Soybean; flowering promoter; gene haplotype; photoperiod.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / genetics
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / physiology
  • CRISPR-Associated Protein 9
  • CRISPR-Cas Systems
  • Cloning, Molecular
  • Flowers / growth & development
  • Gene Editing
  • Gene Expression Regulation, Plant / genetics
  • Genetic Variation / genetics
  • Genetic Variation / physiology
  • Geography
  • Glycine max / genetics
  • Glycine max / growth & development
  • Glycine max / physiology*
  • Photoperiod
  • Plant Proteins / genetics*
  • Plant Proteins / physiology
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Transcriptome

Substances

  • Arabidopsis Proteins
  • FT protein, Arabidopsis
  • Plant Proteins
  • Transcription Factors
  • CRISPR-Associated Protein 9