The knee joint performs a significant amount of positive or negative mechanical work during gradient walking, and targeted assistance during periods of high mechanical work could yield strong human augmentation benefits. This paper explores the biomechanical effects of providing knee extension assistance during the early stance phase of the gait cycle using a powered unilateral knee exoskeleton during gradient walking on able-bodied subjects. Twelve subjects walked on 15% gradient incline and decline surfaces with the exoskeleton providing knee extension assistance during the early stance phase of the gait cycle. For both incline and decline walking, the exoskeleton assistance reduced the muscle activation of the knee extensors on the assisted leg ( ). However, only approximately half the individuals responded to exoskeleton assistance positively by reducing their metabolic cost of walking for both incline and decline tasks. The results indicate that, unlike the individuals who did respond, the individuals who did not respond to the assistance may have penalized their metabolic cost by their biomechanical compensatory behaviors from the unassisted leg.