Immune-profiling of ZIKV-infected patients identifies a distinct function of plasmacytoid dendritic cells for immune cross-regulation

Nat Commun. 2020 May 15;11(1):2421. doi: 10.1038/s41467-020-16217-5.

Abstract

Zika virus (ZIKV) is a mosquito-borne pathogen with increasing public health significance. To characterize immune responses to ZIKV, here we examine transcriptional signatures of CD4 T, CD8 T, B, and NK cells, monocytes, myeloid dendritic cells (mDCs), and plasmacytoid dendritic cells (pDCs) from three individuals with ZIKV infection. While gene expression patterns from most cell subsets display signs of impaired antiviral immune activity, pDCs from infected host have distinct transcriptional response associated with activation of innate immune recognition and type I interferon signaling pathways, but downregulation of key host factors known to support ZIKV replication steps; meanwhile, pDCs exhibit a unique expression pattern of gene modules that are correlated with alternative cell populations, suggesting collaborative interactions between pDCs and other immune cells, particularly B cells. Together, these results point towards a discrete but integrative function of pDCs in the human immune responses to ZIKV infection.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • B-Lymphocytes / virology
  • CD4-Positive T-Lymphocytes / virology
  • CD8-Positive T-Lymphocytes / virology
  • Cells, Cultured
  • Culicidae
  • Dendritic Cells / immunology*
  • Dendritic Cells / virology
  • Female
  • Gene Expression Profiling
  • Humans
  • Immunity, Innate
  • Killer Cells, Natural / virology
  • Leukocytes, Mononuclear / virology
  • Monocytes / metabolism
  • Monocytes / virology
  • Myeloid Cells / virology
  • Transcription, Genetic
  • Virus Replication
  • Zika Virus / immunology
  • Zika Virus Infection / immunology*
  • Zika Virus Infection / virology