Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses

Antiviral Res. 2020 Oct:182:104874. doi: 10.1016/j.antiviral.2020.104874. Epub 2020 Jul 28.

Abstract

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

Keywords: Dengue; Host factor; Positive stranded RNA viruses; SARS-CoV-2; Topoisomerase III-ß (TOP3B).

MeSH terms

  • Betacoronavirus / physiology*
  • Cell Line
  • DNA Topoisomerases, Type I / metabolism*
  • Dengue Virus / physiology
  • Ebolavirus / physiology
  • Gene Knockout Techniques
  • Humans
  • Influenza A virus / physiology
  • RNA Viruses / metabolism*
  • SARS-CoV-2
  • Virus Replication / physiology*
  • Yellow fever virus / physiology
  • Zika Virus / physiology

Substances

  • TOP3B protein, human
  • DNA Topoisomerases, Type I