Perivascular mesenchymal cells control adipose-tissue macrophage accrual in obesity

Nat Metab. 2020 Nov;2(11):1332-1349. doi: 10.1038/s42255-020-00301-7. Epub 2020 Nov 2.

Abstract

Chronic low-grade white adipose tissue (WAT) inflammation is a hallmark of metabolic syndrome in obesity. Here, we demonstrate that a subpopulation of mouse WAT perivascular (PDGFRβ+) cells, termed fibro-inflammatory progenitors (FIPs), activate proinflammatory signalling cascades shortly after the onset of high-fat diet feeding and regulate proinflammatory macrophage accumulation in WAT in a TLR4-dependent manner. FIPs activation in obesity is mediated by the downregulation of zinc-finger protein 423 (ZFP423), identified here as a transcriptional corepressor of NF-κB. ZFP423 suppresses the DNA-binding capacity of the p65 subunit of NF-κB by inducing a p300-to-NuRD coregulator switch. Doxycycline-inducible expression of Zfp423 in PDGFRβ+ cells suppresses inflammatory signalling in FIPs and attenuates metabolic inflammation of visceral WAT in obesity. Inducible inactivation of Zfp423 in PDGFRβ+ cells increases FIP activity, exacerbates adipose macrophage accrual and promotes WAT dysfunction. These studies implicate perivascular mesenchymal cells as important regulators of chronic adipose-tissue inflammation in obesity and identify ZFP423 as a transcriptional break on NF-κB signalling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adipose Tissue, White / pathology*
  • Animals
  • DNA-Binding Proteins / metabolism
  • Diet, High-Fat
  • Hypoglycemic Agents / pharmacology
  • Insulin / pharmacology
  • Macrophages / pathology*
  • Mesenchymal Stem Cells*
  • Mice
  • Mice, Inbred C57BL
  • Obesity / pathology*
  • Receptor, Platelet-Derived Growth Factor beta / metabolism
  • Signal Transduction
  • Toll-Like Receptor 4 / metabolism
  • Transcription Factor RelA / metabolism
  • Transcription Factors / metabolism

Substances

  • DNA-Binding Proteins
  • Ebfaz protein, mouse
  • Hypoglycemic Agents
  • Insulin
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4
  • Transcription Factor RelA
  • Transcription Factors
  • Receptor, Platelet-Derived Growth Factor beta