Extraction-Free Methods for the Detection of SARS-CoV-2 by Reverse Transcription-PCR: a Comparison with the Cepheid Xpert Xpress SARS-CoV-2 Assay across Two Medical Centers

J Clin Microbiol. 2021 Jan 21;59(2):e02643-20. doi: 10.1128/JCM.02643-20. Print 2021 Jan 21.

Authors

Andrew Cameron¹, Nicole D Pecora^#^{1

2}, Matthew A Pettengill^#³

Affiliations

¹ University of Rochester Medical Center, Clinical Microbiology, Department of Pathology and Laboratory Medicine, Rochester, New York, USA.
² University of Rochester Medical Center, Department of Microbiology and Immunology, Rochester, New York, USA.
³ Thomas Jefferson University Hospital, Department of Pathology, Anatomy, and Cell Biology, Philadelphia, Pennsylvania, USA [email protected].

^# Contributed equally.

No abstract available

Keywords: COVID-19; RT-PCR; SARS-CoV-2; extraction.

Publication types

Comparative Study
Letter
Multicenter Study

MeSH terms

COVID-19 / diagnosis*
COVID-19 Nucleic Acid Testing / economics
COVID-19 Nucleic Acid Testing / methods*
Humans
Nasopharynx / virology
SARS-CoV-2 / genetics
SARS-CoV-2 / isolation & purification*
Sensitivity and Specificity
Specimen Handling