Data on proteomic analysis of milk extracellular vesicles from bovine leukemia virus-infected cattle

Md Matiur Rahman; Yassien Badr; Yuji O Kamatari; Yuko Kitamura; Kaori Shimizu; Ayaka Okada; Yasuo Inoshima

doi:10.1016/j.dib.2020.106510

Data on proteomic analysis of milk extracellular vesicles from bovine leukemia virus-infected cattle

Data Brief. 2020 Nov 10:33:106510. doi: 10.1016/j.dib.2020.106510. eCollection 2020 Dec.

Authors

Md Matiur Rahman^{1

2

3}, Yassien Badr^{2

4}, Yuji O Kamatari⁵, Yuko Kitamura⁶, Kaori Shimizu², Ayaka Okada^{2

7}, Yasuo Inoshima^{1

2

8

7}

Affiliations

¹ The United Graduate School of Veterinary Sciences, Gifu University, Japan.
² Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, Japan.
³ Department of Medicine, Faculty of Veterinary, Animal, and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.
⁴ Department of Animal Medicine, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt.
⁵ Division of Instrumental Analysis, Life Science Research Center, Gifu University, Japan.
⁶ Gifu Prefectural Chuo Livestock Hygiene Service Center, Japan.
⁷ Joint Graduate School of Veterinary Sciences, Gifu University, Japan.
⁸ Education and Research Center for Food Animal Health, Gifu University (GeFAH), Japan.

Abstract

Milk extracellular vesicles (EVs) are nanoparticles that contain proteins, mRNAs, microRNAs, DNAs, and lipids that involved in several biological functions. Milk EVs provide proteins that could represent relevant novel biomarkers for monitoring of different diseases such as breast cancer and mastitis in humans and animals, respectively. Bovine leukemia virus (BLV) is an oncogenic virus that causes progressive B-cell lymphosarcoma in cattle. Here, we aimed to identify proteins in milk EVs from BLV-infected cattle compared with those from uninfcetd cattle. Proteomic analysis was performed by using a comprehensive nano liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) approach. Identified proteins were analyzed by using a proteomic software, Scaffold-Data Independent Acquisition (Scaffold-DIA).

Keywords: Bovine leukemia virus; Milk extracellular vesicles; NanoLC-MS/MS; Proteomic analysis; Scaffold-DIA.