Intradermal delivery of receptor-binding domain of SARS-CoV-2 spike protein with dissolvable microneedles to induce humoral and cellular responses in mice

Bioeng Transl Med. 2020 Dec 12;6(1):e10202. doi: 10.1002/btm2.10202. eCollection 2021 Jan.

Abstract

The S1 subunit of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein contains an immunogenic receptor-binding domain (RBD), which is a promising candidate for the development of a potential vaccine. This study demonstrated that intradermal delivery of an S-RBD vaccine using a dissolvable microneedle skin patch can induce both significant B-cell and significant T-cell responses against S-RBD. Importantly, the outcomes were comparable to that of conventional bolus injection.

Keywords: COVID‐19; SARS‐CoV‐2; intradermal delivery; microneedle; receptor‐binding domain; spike protein.