Chloroplast genome and phylogenetic analyses of Poncirus trifoliata (Rutaceae)

Shui-Lian He; Yang Tian; Yang Yang; Chong-Ying Shi

doi:10.1080/23802359.2019.1687023

Chloroplast genome and phylogenetic analyses of Poncirus trifoliata (Rutaceae)

Mitochondrial DNA B Resour. 2020 Jun 1;5(3):2205-2206. doi: 10.1080/23802359.2019.1687023. eCollection 2020.

Authors

Shui-Lian He¹, Yang Tian², Yang Yang^{2

3}, Chong-Ying Shi⁴

Affiliations

¹ College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.
² Yunnan Key Laboratory of Biomass Big Data, Yunnan Agricultural University, Kunming, China.
³ College of Science, Yunnan Agricultural University, Kunming, China.
⁴ Institute of Food Science and Technology, Yunnan Agricultural University, Yunnan, China.

Abstract

Poncirus trifoliata is an important medicinal plant that is used to treat human diseases. In this study, the complete chloroplast (cp) genome of P. trifoliata was assembled based on the Illumina sequencing reads. The cp genome of P. trifoliata was 160,260 bp and contained two short inverted repeat regions (27,029 bp) which were separated by a small single copy region (18,760 bp) and a large single copy region (87,442 bp). The cp genome encodes 113 unique genes, including 79 protein-coding genes, 30 transfer RNA genes and 4 ribosomal RNA genes. The topology of the phylogenetic tree showed that P. trifoliata is closely clustered with genus Citrus.

Keywords: Poncirus trifoliata; chloroplast genome; medicinal plant; phylogenetic analysis.

Grants and funding

This work was supported by Chinese Cassava Agro-technology Research System [CARS-11-YNSJ], Yunnan Provincial Key Laboratory of Biological Big Data [2018DG024]; and the Science and Technology Plan Projects of Yunnan Province [2016FD024]