Interfacial Self-Assembly of Heparin-Mimetic Multilayer on Membrane Substrate as Effective Antithrombotic, Endothelialization, and Antibacterial Coating

In this study, we design the interfacial self-assembly of heparin-mimetic multilayer on poly(ether sulfone) (PES) membrane, which can endow the substrate with excellent cytocompatibility, highly hemocompatibility and enhanced antibacterial properties. The coated 3D sponge-like multilayer was fabricated by surface engineered layer by layer assembly of sulfonic amino polyether sulfone (SNPES) and quaternized chitosan (QC). The cell morphology observation and viability evaluation suggested that the assembled multilayer coating had remarkable cytocompatibility with endothelial cells due to the synergistic promotion of bovine serum albumin adsorption and heparin-mimetic groups; which further indicated that surface endothelialization could be achieved on the heparin-mimetic multilayer. The systematical tests of antithrombotic and blood activation indicated that the heparin-mimetic multilayer-coated membrane owned significantly suppressed adsorption of bovine serum fibrinogen, platelet adhesion and activation, prolonged clotting times, as well as lower activation of blood complement. Furthermore, the antibacterial test suggested the multilayer coated substrates exhibited obvious inhibition capability for both Escherichia coli and Staphylococcus aureus. Therefore, we believe that the developed SNPES/QC multilayer on PES membrane show great potential as a multifunctional coating toward versatile biomedical applications due to the integrated and highly effective antithrombotic, endothelialization, and antibacterial properties.