We elucidated the proper routes for injecting lipid-DNA micelles formulated with an oligonucleotide immune adjuvant in naïve and tumor-bearing mice. We report herein that after assessing six ways of administering an immunostimulatory nanoparticle (INP), several of the injection routes effectively induced dendritic cell-mediated immune-stimulation in vivo, making them amenable to cancer immunotherapies and vaccines using such DNA materials.
Keywords: DNA nanotechnology; adjuvants; dendritic cells; hybrid materials; micelles; nucleic acids.