Activation of GPR37 in macrophages confers protection against infection-induced sepsis and pain-like behaviour in mice

Nat Commun. 2021 Mar 17;12(1):1704. doi: 10.1038/s41467-021-21940-8.

Abstract

GPR37 was discovered more than two decades ago, but its biological functions remain poorly understood. Here we report a protective role of GPR37 in multiple models of infection and sepsis. Mice lacking Gpr37 exhibited increased death and/or hypothermia following challenge by lipopolysaccharide (LPS), Listeria bacteria, and the mouse malaria parasite Plasmodium berghei. Sepsis induced by LPS and Listeria in wild-type mice is protected by artesunate (ARU) and neuroprotectin D1 (NPD1), but the protective actions of these agents are lost in Gpr37-/- mice. Notably, we found that ARU binds to GPR37 in macrophages and promotes phagocytosis and clearance of pathogens. Moreover, ablation of macrophages potentiated infection, sepsis, and their sequelae, whereas adoptive transfer of NPD1- or ARU-primed macrophages reduced infection, sepsis, and pain-like behaviors. Our findings reveal physiological actions of ARU in host cells by activating macrophages and suggest that GPR37 agonists may help to treat sepsis, bacterial infections, and malaria.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Artesunate / metabolism
  • Artesunate / pharmacology
  • Artesunate / therapeutic use
  • Disease Models, Animal
  • Docosahexaenoic Acids / metabolism
  • Docosahexaenoic Acids / pharmacology
  • Docosahexaenoic Acids / therapeutic use
  • Lipopolysaccharides / toxicity
  • Listeria monocytogenes / pathogenicity
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Macrophages / pathology
  • Macrophages / transplantation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Docking Simulation
  • Pain / immunology
  • Pain / mortality
  • Pain / prevention & control*
  • Phagocytosis / drug effects
  • Plasmodium berghei / pathogenicity
  • Receptors, G-Protein-Coupled / deficiency
  • Receptors, G-Protein-Coupled / metabolism*
  • Sepsis / immunology
  • Sepsis / mortality
  • Sepsis / prevention & control*
  • Sepsis / therapy

Substances

  • Gpr37 protein, mouse
  • Lipopolysaccharides
  • Receptors, G-Protein-Coupled
  • protectin D1
  • Docosahexaenoic Acids
  • Artesunate