MicroELISA detection of thymosin alpha 1 released in thymic organ cultures

J Immunol Methods. 1988 Jun 13;110(2):261-5. doi: 10.1016/0022-1759(88)90112-3.

Abstract

Using a polyclonal specific rabbit anti-thymosin alpha 1 a highly sensitive enzyme-linked immunosorbent assay (ELISA) has been developed to measure thymosin alpha 1. Production of thymosin alpha 1 was detected in both thymic organ cultures and in mouse serum. The method is rapid (5 h), reproducible and easy to perform.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Antibody Reactions
  • Enzyme-Linked Immunosorbent Assay*
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Organ Culture Techniques
  • Spleen / metabolism
  • Thymalfasin
  • Thymosin / analogs & derivatives*
  • Thymosin / analysis
  • Thymosin / immunology
  • Thymosin / metabolism
  • Thymus Gland / cytology
  • Thymus Gland / metabolism*

Substances

  • Thymosin
  • Thymalfasin