[Development and validation of a diagnostic model of antimicrobial resistance by extended-spectrum beta-lactamases in community-acquired urinary tract infections. How to adjust the prediction in variable outcome prevalences? The usefulness of contracting predictors with LASSO regression]

Rev Chilena Infectol. 2020 Dec;37(6):683-689. doi: 10.4067/S0716-10182020000600683.
[Article in Spanish]

Abstract

Background: In the case of community-acquired urinary tract infection, the identification of Enterobacteriaceae with extended spectrum beta-lactamases (ESBL) can optimize treatment, control and follow-up strategies, however the effect of variable prevalences of this resistance pattern has affected the external validity of this type of models.

Aim: To develop a diagnostic predictive model that adjusts the prediction error in variable prevalences using the LASSO regression.

Methods: A diagnostic predictive model of community-acquired urinary tract infection by infection by ESBL producing Enterobacteriaceae was designed. A cross-sectional study was used for both construction and validation. To assess the effect of the variable prevalence of the outcome, the validation was performed with a population in which the proportion of isolates with this resistance mechanism was lower, the participants were adult patients who consulted the emergency services of two medium-level hospital institutions. complexity of the city of Medellin. To adjust for the effect of an environment with a lower proportion of antimicrobial resistance, we used the contraction of predictors by LASSO regression.

Results: 303 patients were included for the construction of the model, six predictors were evaluated and validation was carried out in 220 patients.

Conclusion: The adjusted model with LASSO regression favored the external validity of the model in populations with a proportion of ESBL producing isolates in urine culture of outpatients between 11 and 16%. This study provides criteria for early isolation when predictors are present in populations with proportions of resistance in ambulatory urine cultures close to 15% and proposes a methodology for the adjustment of errors in the design of prediction models for antimicrobial resistance.

MeSH terms

  • Adult
  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use
  • Community-Acquired Infections* / drug therapy
  • Cross-Sectional Studies
  • Drug Resistance, Bacterial
  • Enterobacteriaceae Infections* / drug therapy
  • Enterobacteriaceae Infections* / epidemiology
  • Humans
  • Microbial Sensitivity Tests
  • Prevalence
  • Urinary Tract Infections* / drug therapy
  • Urinary Tract Infections* / epidemiology
  • beta-Lactamases

Substances

  • Anti-Bacterial Agents
  • beta-Lactamases