The stimulator of interferon genes (STING) pathway is upregulated in striatal astrocytes of patients with multiple system atrophy

Neurosci Lett. 2021 Jul 13:757:135972. doi: 10.1016/j.neulet.2021.135972. Epub 2021 May 24.

Abstract

Multiple system atrophy (MSA) is a progressive neurodegenerative disorder characterized by the accumulation of pathogenic phosphorylated α-synuclein in oligodendrocytes. In brains affected by MSA, severe astrogliosis is also observed, but its precise role in MSA pathogenesis remains largely unknown. Recently, the stimulator of interferon genes (STING) pathway and type I interferons, its downstream molecules, have been reported to be involved in the neurodegenerative process and to be activated in astrocytes. This study aimed to investigate the role of the STING pathway in the pathogenesis of MSA using postmortem brains. Samples used for immunohistochemical analysis included 6 cases of MSA parkinsonism type (MSA-P), 6 cases of MSA cerebellar type (MSA-C), and 7 age-matched controls. In MSA-P cases, astrocytes immunopositive for STING and TANK-binding kinase 1 (TBK1), its downstream molecule, were abundantly observed in the putamen and the substantia nigra. Moreover, these molecules colocalized with glial fibrillary acidic protein (GFAP) in reactive astrocytes, and the density of STING-positive astrocytes correlated with that of GFAP-positive reactive astrocytes in the brains of patients with MSA-P. These results suggest that the upregulated expression of STING pathway-related proteins in astrocytes and the subsequent inflammation may contribute to the pathogenesis in MSA-P and could provide novel therapeutic targets for the treatment of MSA.

Keywords: Astrocyte; Multiple system atrophy; Stimulator of interferon genes; TANK-binding kinase 1; Type I interferons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Astrocytes / metabolism*
  • Case-Control Studies
  • Glial Fibrillary Acidic Protein / analysis
  • Glial Fibrillary Acidic Protein / metabolism
  • Humans
  • Male
  • Membrane Proteins / analysis
  • Membrane Proteins / metabolism*
  • Middle Aged
  • Multiple System Atrophy / immunology*
  • Multiple System Atrophy / pathology
  • Putamen / cytology
  • Putamen / immunology
  • Putamen / pathology*
  • Signal Transduction / immunology
  • Substantia Nigra / cytology
  • Substantia Nigra / immunology
  • Substantia Nigra / pathology*
  • Up-Regulation / immunology

Substances

  • GFAP protein, human
  • Glial Fibrillary Acidic Protein
  • Membrane Proteins
  • STING1 protein, human