Estimation of renal perfusion based on measurement of rubidium-82 clearance by PET/CT scanning in healthy subjects

Background: Changes in renal blood flow (RBF) may play a pathophysiological role in hypertension and kidney disease. However, RBF determination in humans has proven difficult. We aimed to confirm the feasibility of RBF estimation based on positron emission tomography/computed tomography (PET/CT) and rubidium-82 (⁸²Rb) using the abdominal aorta as input function in a 1-tissue compartment model.

Methods: Eighteen healthy subjects underwent two dynamic ⁸²Rb PET/CT scans in two different fields of view (FOV). FOV-A included the left ventricular blood pool (LVBP), the abdominal aorta (AA) and the majority of the kidneys. FOV-B included AA and the kidneys in their entirety. In FOV-A, an input function was derived from LVBP and from AA, in FOV-B from AA. One-tissue compartmental modelling was performed using tissue time activity curves generated from volumes of interest (VOI) contouring the kidneys, where the renal clearance of ⁸²Rb is represented by the K₁ kinetic parameter. Total clearance for both kidneys was calculated by multiplying the K₁ values with the volume of VOIs used for analysis. Intra-assay coefficients of variation and inter-observer variation were calculated.

Results: For both kidneys, K₁ values derived from AA did not differ significantly from values obtained from LVBP, neither were significant differences seen between AA in FOV-A and AA in FOV-B, nor between the right and left kidneys. For both kidneys, the intra-assay coefficients of variation were low (~ 5%) for both input functions. The measured K₁ of 2.80 ml/min/cm³ translates to a total clearance for both kidneys of 766 ml/min/1.73 m².

Conclusion: Measurement of renal perfusion based on PET/CT and ⁸²Rb using AA as input function in a 1-tissue compartment model is feasible in a single FOV. Based on previous studies showing ⁸²Rb to be primarily present in plasma, the measured K₁ clearance values are most likely representative of effective renal plasma flow (ERPF) rather than estimated RBF values, but as the accurate calculation of total clearance/flow is very much dependent on the analysed volume, a standardised definition for the employed renal volumes is needed to allow for proper comparison with standard ERPF and RBF reference methods.