High-Quality Genome Reconstruction of Candida albicans CHN1 Using Nanopore and Illumina Sequencing and Hybrid Assembly

Shipra Garg; Piyush Ranjan; John R Erb-Downward; Gary B Huffnagle

doi:10.1128/MRA.00299-21

High-Quality Genome Reconstruction of Candida albicans CHN1 Using Nanopore and Illumina Sequencing and Hybrid Assembly

Microbiol Resour Announc. 2021 Jun 10;10(23):e0029921. doi: 10.1128/MRA.00299-21. Epub 2021 Jun 10.

Authors

Shipra Garg¹, Piyush Ranjan², John R Erb-Downward², Gary B Huffnagle^{1

3

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Affiliations

¹ Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA.
² Division of Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, Michigan, USA.
³ Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.
⁴ Mary H. Weiser Food Allergy Center, University of Michigan, Ann Arbor, Michigan, USA.

Abstract

We report an improved, nearly closed, high-quality draft genome reconstruction of the Candida albicans CHN1 strain (ATCC MYA-4779), a human isolate, using Illumina and Nanopore sequencing. Covering six complete and two partial nuclear chromosomes along with a partial mitochondrial genome, this assembly is 14,787,852 bases in size, with 5,935 genes.

Abstract

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