Genome sequence of the coffee root-knot nematode Meloidogyne exigua

Ngan Thi Phan; Guillaume Besnard; Rania Ouazahrou; William Solano Sánchez; Lisa Gil; Sophie Manzi; Stéphane Bellafiore

doi:10.21307/jofnem-2021-065

Genome sequence of the coffee root-knot nematode Meloidogyne exigua

J Nematol. 2021 Jul 19:53:e2021-65. doi: 10.21307/jofnem-2021-065. eCollection 2021.

Authors

Ngan Thi Phan¹, Guillaume Besnard², Rania Ouazahrou³, William Solano Sánchez⁴, Lisa Gil⁵, Sophie Manzi², Stéphane Bellafiore¹

Affiliations

¹ PHIM Plant Health Institute, University of Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, France.
² CNRS-UPS-IRD, UMR5174, EDB, 118 route de Narbonne, Université Paul Sabatier, 31062 Toulouse, France.
³ University of Rennes 1, UFR SVE, 35065, Rennes, France.
⁴ CATIE - Centro Agronómico Tropical de Investigación y Enseñanza, Turrialba, Costa Rica.
⁵ US 1426, GeT-PlaGe, Genotoul, INRAE, Castanet-Tolosan, France.

Abstract

Root-knot nematodes (Meloidogyne spp.) cause serious damages on most crops. Here, we report a high-quality genome sequence of Meloidogyne exigua (population Mex1, Costa Rica), a major pathogen of coffee. Its mitogenome (20,974 bp) was first assembled and annotated. The nuclear genome was then constructed consisting of 206 contigs, with an N50 length of 1.89 Mb and a total assembly length of 42.1 Mb.

Keywords: Genomics; Illumina; Mitogenome; Nanopore sequencing; Nuclear genome; Root-knot nematode.