Rapid Detection of Carbapenemase-producing Enterobacteriaceae From Blood Culture Bottles of Known CPE Carriers: Real-world Experience

Pediatr Infect Dis J. 2022 Jan 1;41(1):45-47. doi: 10.1097/INF.0000000000003311.

Abstract

We used a rapid antigen test for the detection of carbapenemases directly from positive blood culture bottles of pediatric hemato-oncologic patients, known carriers of carbapenemase-producing enterobacteriaceae. Resistance mechanism was detected within 15 minutes of observing Gram-negative bacilli from a positive bottle, leading to treatment modification. This simple-to-use, inexpensive assay shortens the interval between empiric to tailored antimicrobial therapy.

MeSH terms

  • Adolescent
  • Anti-Bacterial Agents / pharmacology
  • Antigens, Bacterial / blood*
  • Bacterial Proteins / analysis
  • Bacterial Proteins / biosynthesis*
  • Blood Culture / economics
  • Blood Culture / methods
  • Carbapenem-Resistant Enterobacteriaceae / classification
  • Carbapenem-Resistant Enterobacteriaceae / drug effects
  • Carbapenem-Resistant Enterobacteriaceae / enzymology*
  • Carbapenem-Resistant Enterobacteriaceae / isolation & purification*
  • Child
  • Child, Preschool
  • Chromatography, Affinity / economics
  • Chromatography, Affinity / instrumentation
  • Chromatography, Affinity / methods*
  • Chromatography, Affinity / standards
  • Enterobacteriaceae Infections / diagnosis
  • Enterobacteriaceae Infections / microbiology*
  • Female
  • Humans
  • Infant
  • Male
  • Microbial Sensitivity Tests
  • Sensitivity and Specificity
  • beta-Lactamases / analysis
  • beta-Lactamases / biosynthesis*

Substances

  • Anti-Bacterial Agents
  • Antigens, Bacterial
  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase