Generation and transmission of interlineage recombinants in the SARS-CoV-2 pandemic

Cell. 2021 Sep 30;184(20):5179-5188.e8. doi: 10.1016/j.cell.2021.08.014. Epub 2021 Aug 17.

Abstract

We present evidence for multiple independent origins of recombinant SARS-CoV-2 viruses sampled from late 2020 and early 2021 in the United Kingdom. Their genomes carry single-nucleotide polymorphisms and deletions that are characteristic of the B.1.1.7 variant of concern but lack the full complement of lineage-defining mutations. Instead, the remainder of their genomes share contiguous genetic variation with non-B.1.1.7 viruses circulating in the same geographic area at the same time as the recombinants. In four instances, there was evidence for onward transmission of a recombinant-origin virus, including one transmission cluster of 45 sequenced cases over the course of 2 months. The inferred genomic locations of recombination breakpoints suggest that every community-transmitted recombinant virus inherited its spike region from a B.1.1.7 parental virus, consistent with a transmission advantage for B.1.1.7's set of mutations.

Keywords: B.1.1.7; SARS-CoV-2; evolution; genomic epidemiology; genomics; recombination; variants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence / genetics
  • COVID-19 / epidemiology*
  • COVID-19 / transmission*
  • COVID-19 / virology
  • Computational Biology / methods
  • Gene Frequency
  • Genome, Viral
  • Genotype
  • Humans
  • Mutation
  • Pandemics*
  • Phylogeny
  • Polymorphism, Single Nucleotide
  • Recombination, Genetic*
  • SARS-CoV-2 / genetics*
  • United Kingdom / epidemiology
  • Whole Genome Sequencing / methods