Abstract
Angelman syndrome (AS) is a neurogenetic disorder characterized by severe developmental delay with absence of speech, happy disposition, frequent laughter, hyperactivity, stereotypies, ataxia and seizures with specific EEG abnormalities. There is a 10-15% of patients with an AS phenotype whose genetic cause remains unknown (Angelman-like syndrome, AS-like). Whole-exome sequencing (WES) was performed on a cohort of 14 patients with clinical features of AS and no molecular diagnosis. As a result, we identified 10 de novo and 1 X-linked pathogenic/likely pathogenic variants in 10 neurodevelopmental genes (SYNGAP1, VAMP2, TBL1XR1, ASXL3, SATB2, SMARCE1, SPTAN1, KCNQ3, SLC6A1 and LAS1L) and one deleterious de novo variant in a candidate gene (HSF2). Our results highlight the wide genetic heterogeneity in AS-like patients and expands the differential diagnosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Angelman Syndrome / genetics*
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Child
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Exome Sequencing / methods*
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Female
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Gene Regulatory Networks*
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Genetic Association Studies
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Genetic Predisposition to Disease
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Heat-Shock Proteins
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Humans
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Infant
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Male
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Matrix Attachment Region Binding Proteins / genetics
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Receptors, Cytoplasmic and Nuclear / genetics
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Repressor Proteins / genetics
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Transcription Factors / genetics
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Vesicle-Associated Membrane Protein 2 / genetics
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Young Adult
Substances
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ASXL3 protein, human
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Heat-Shock Proteins
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Matrix Attachment Region Binding Proteins
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Receptors, Cytoplasmic and Nuclear
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Repressor Proteins
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SATB2 protein, human
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TBL1XR1 protein, human
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Transcription Factors
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VAMP2 protein, human
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Vesicle-Associated Membrane Protein 2
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HSF2 protein, human
Grants and funding
This work is supported by Instituto de Salud Carlos III (MG, PI16/01411), Asociación Española de Síndrome de Angelman (EG), Institut d’investigació i innovació Parc Taulí I3PT (CA, CIR2016/025, CIR2018/021) and Ministerio de Economía y Competitividad (XD, SAF2016-14 80255-R). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.