Clinicopathological features and resistance mechanisms in HIP1-ALK-rearranged lung cancer: A multicenter study

Genes Chromosomes Cancer. 2022 Apr;61(4):177-186. doi: 10.1002/gcc.23005. Epub 2021 Nov 1.

Abstract

Anaplastic lymphoma kinase (ALK)-rearranged non-small cell lung cancer (NSCLC) respond well to ALK tyrosine kinase inhibitors (TKIs), and echinoderm microtubule-associated protein-like 4 (EML4)-ALK-rearranged NSCLC accounts for the majority of those patients. However, few studies have evaluated ALK-TKIs treatment for patients with huntingtin-interacting protein 1 (HIP1)-ALK fusions. This retrospective study evaluated the clinicopathological characteristics, genomic features, response to ALK-TKIs, and resistance mechanisms in 11 cases with HIP1-ALK fusions from five Chinese centers. Patients who received crizotinib at the Chinese centers had an objective response rate of 90% [9/10 cases, 95% confident index (CI): 54.1%-99.5%], median progression-free survival of 17.9 months (95% CI: 5.8-NA months), and median overall survival of 58.8 months (95% CI: 24.7-NA months). One patient who received first-line lorlatinib treatment achieved partial response for > 26.5 months. Despite the small sample size, HIP1-ALK (H21:A20) variant was the most common variant (four of 11 cases, 36.4%) and associated with better outcomes. Among the 11 cases, there were eight patients having available specimens for genetic testing before ALK-TKIs treatment and four patients undergoing biopsy after ALK-TKIs failure. The most common coexisting gene was TP53 among 11 patients and two of four patients after crizotinib failure harbored acquired ALK mutations (e.g., L1152V/Q1146K and L1196M). Brigatinib treatment appeared to be effective for a patient who failed crizotinib treatment because of the L1152V/Q1146K mutations, which might be related to increased binding affinity to these mutants. Although HIP1-ALK-rearranged NSCLC appears to initially respond well to ALK-TKIs, crizotinib resistance may be correlated with the AKAP9-BRAF fusion, ALK compound mutations (L1152V/Q1146K), and the ALK L1196M mutation. Larger studies are needed to evaluate the significance of HIP1-ALK-rearranged NSCLC.

Keywords: HIP1-ALK rearrangement; clinicopathological and genomic features; mechanisms of drug-resistance; nonsmall cell lung cancer; tyrosine kinases inhibitors.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activin Receptors, Type II
  • Adult
  • Aged
  • Anaplastic Lymphoma Kinase / genetics*
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Crizotinib / therapeutic use
  • DNA-Binding Proteins / genetics*
  • Drug Resistance, Neoplasm*
  • Female
  • Gene Rearrangement*
  • Humans
  • Immunoglobulin Fc Fragments
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Male
  • Middle Aged
  • Oncogene Proteins, Fusion / genetics*
  • Protein Kinase Inhibitors / therapeutic use
  • Recombinant Fusion Proteins
  • Retrospective Studies
  • Survival Analysis

Substances

  • DNA-Binding Proteins
  • HIP1 protein, human
  • Immunoglobulin Fc Fragments
  • Oncogene Proteins, Fusion
  • Protein Kinase Inhibitors
  • Recombinant Fusion Proteins
  • Crizotinib
  • Anaplastic Lymphoma Kinase
  • Activin Receptors, Type II
  • ALK1-Fc fusion protein, human