Meta-analyses identify DNA methylation associated with kidney function and damage

Nat Commun. 2021 Dec 9;12(1):7174. doi: 10.1038/s41467-021-27234-3.

Abstract

Chronic kidney disease is a major public health burden. Elevated urinary albumin-to-creatinine ratio is a measure of kidney damage, and used to diagnose and stage chronic kidney disease. To extend the knowledge on regulatory mechanisms related to kidney function and disease, we conducted a blood-based epigenome-wide association study for estimated glomerular filtration rate (n = 33,605) and urinary albumin-to-creatinine ratio (n = 15,068) and detected 69 and seven CpG sites where DNA methylation was associated with the respective trait. The majority of these findings showed directionally consistent associations with the respective clinical outcomes chronic kidney disease and moderately increased albuminuria. Associations of DNA methylation with kidney function, such as CpGs at JAZF1, PELI1 and CHD2 were validated in kidney tissue. Methylation at PHRF1, LDB2, CSRNP1 and IRF5 indicated causal effects on kidney function. Enrichment analyses revealed pathways related to hemostasis and blood cell migration for estimated glomerular filtration rate, and immune cell activation and response for urinary albumin-to-creatinineratio-associated CpGs.

Publication types

  • Meta-Analysis
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • CpG Islands
  • DNA Methylation*
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Interferon Regulatory Factors / genetics
  • Interferon Regulatory Factors / metabolism
  • Kidney / metabolism
  • Kidney / physiopathology
  • Kidney Function Tests
  • LIM Domain Proteins / genetics
  • LIM Domain Proteins / metabolism
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Middle Aged
  • Renal Insufficiency, Chronic / genetics*
  • Renal Insufficiency, Chronic / metabolism
  • Renal Insufficiency, Chronic / physiopathology
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • IRF5 protein, human
  • Interferon Regulatory Factors
  • LDB2 protein, human
  • LIM Domain Proteins
  • Membrane Proteins
  • PHD and RING finger domain-containing protein 1, human
  • Transcription Factors