Effects of diesel-engine exhaust emissions on seed germination and seedling growth of Brassicaceae family using digital image analysis

This paper describes a multi-phase investigation into the direct effect of diesel exhaust emission on seed germination traits and biochemical changes responsible for observed effects in seeds belongs to the Brassica family. Diesel exhaust emissions were collected in germination boxes and seeds were exposed to diesel exhaust pollutants for durations of 30 to 120 min with 30 min intervals. Observed effects include seed germination inhibition, changes in seeds' antioxidants activity, and protein content. The lowest seed germination of canola (71 %) and arugula (84 %) was observed when seeds were exposed to 120 min of diesel exhaust pollution. Seed exposure to diesel exhaust emission for 60 min, caused a 23 % and 8 % decline of germination index of canola and arugula, respectively. The maximum seed soluble protein for canola (3.72 mg/g FW) was observed in seeds exposed to 120 min diesel exhaust pollution declined to 1.65 mg/g FW, and 0.60 mg/g FW after 60 and 30 min exposure to diesel exhaust, respectively. The maximum protein content of arugula seeds (0.95 mg/g FW) was observed in the control treatment and it was reduced to 0.72 mg/g FW and 0.53 mg/g FW after 60 and 90 min exposure to diesel exhaust pollution. Catalase activity was significantly reduced as canola seed exposure to diesel exhausted was increased while there were no statistically significant changes for catalase activity of arugula seeds. All evidence suggested that time of exposure was the key phytotoxic component of exhaust emissions, and highlights the potential for detrimental effects of vehicle emissions on agro-ecosystems.