Induction of T Cell Responses by Vaccination of a Streptococcus pneumoniae Whole-Cell Vaccine

Methods Mol Biol. 2022:2410:345-355. doi: 10.1007/978-1-0716-1884-4_17.

Abstract

The induction of T cell responses by vaccination is important for protection against infection. We have previously shown that immunization with a killed Streptococcus pneumoniae whole-cell vaccine (SPWCV) by either intranasal immunization or subcutaneous immunization induced T cell responses to SPWCV. Protection against colonization by S. pneumoniae is dependent on CD4+ IL-17A production induced by immunization. Here, we present detailed protocols for preparation of SPWCV, immunization of mice, and assay for T cell responses in blood and splenocytes in immunized mice.

Keywords: Immunization; Streptococcus pneumoniae; T cell; Vaccine.

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antibodies, Bacterial
  • Mice
  • Pneumococcal Infections* / prevention & control
  • Pneumococcal Vaccines
  • Streptococcus pneumoniae* / immunology
  • T-Lymphocytes / immunology
  • Vaccination

Substances

  • Antibodies, Bacterial
  • Pneumococcal Vaccines