One- and Two-Photon Uncaging of Carbon Monoxide (CO) with Real-Time Monitoring: On-Demand Carbazole-Based Dual CO-Releasing Platform to Test over Single and Combinatorial Approaches for the Efficient Regression of Orthotopic Murine Melanoma In Vivo

J Med Chem. 2022 Feb 10;65(3):1822-1834. doi: 10.1021/acs.jmedchem.1c00750. Epub 2022 Jan 12.

Abstract

Herein, we report three new metal-free, photochemically active single, dual, and combinatorial CORMs (photoCORMs) based on a carbazole-fused 1,3-dioxol-2-one moiety which released one equivalent of CO, two equivalent of CO, and a combination of one equivalent of each CO and anticancer drug upon one- and two-photon excitation, respectively. The photoCORMs exhibited good cellular uptake and real-time monitoring ability of CO uncaging by a color change approach in cancerous B16F10 cells. Interestingly, the cytotoxicity assay on B16F10 cells indicated that the dual photoCORM has increased anticancer activity over the single and combinatorial photoCORMs upon irradiation. Our results also showed that CO could accelerate the effectiveness of the well-known anticancer drug (chlorambucil). Finally, the in vivo evaluation of the dual photoCORM on an established murine melanoma tumor (C57BL/6J mouse model) manifested a significant regression of tumor volume and led to significant improvement (>50%) in the overall survivability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / radiation effects
  • Antineoplastic Agents / therapeutic use*
  • Apoptosis / drug effects
  • Carbazoles / chemical synthesis
  • Carbazoles / radiation effects
  • Carbazoles / therapeutic use*
  • Carbon Monoxide / metabolism*
  • Cell Line, Tumor
  • Dioxoles / chemical synthesis
  • Dioxoles / radiation effects
  • Dioxoles / therapeutic use*
  • Female
  • Infrared Rays
  • Melanoma / drug therapy*
  • Mice
  • Mice, Inbred C57BL
  • Photons

Substances

  • Antineoplastic Agents
  • Carbazoles
  • Dioxoles
  • Carbon Monoxide