Differential miRNA expression profiling of highly pathogenic avian influenza virus H5N1 infected chicken lungs reveals critical microRNAs, biological pathways and genes involved in the molecular pathogenesis

Virol Sin. 2022 Jun;37(3):465-468. doi: 10.1016/j.virs.2022.03.004. Epub 2022 Mar 9.

¹ Pathogenomics Laboratory, ICAR-National Institute of High Security Animal Diseases, OIE referral laboratory for Avian Influenza, Bhopal 462 021, India. Electronic address: [email protected].
² Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Bareilly 243 122, India.
³ ICAR-National Institute of High Security Animal Diseases, OIE referral lab for Avian Influenza, Bhopal 462 021, India.
⁴ Pathogenomics Laboratory, ICAR-National Institute of High Security Animal Diseases, OIE referral laboratory for Avian Influenza, Bhopal 462 021, India.

Abstract

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Highthrouput sequencing of small RNA of H5N1 infected and mock infected chicken lungs.
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297 miRNAs identified in mock-infected and 201 miRNAs identified in AIV infected chicken lungs.
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36 miRNAs were upregulated and 90 were downregulated during H5N1 infection.
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Functional analysis and gene ontology of predicted target genes of expressed miRNAs.
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MAPK pathway, NF-κB, IGF and gga-let-7b might play important role during H5N1 pathogenesis.