Tracking metabolites at single-cell resolution reveals metabolic dynamics during plant mitosis

Emiko Okubo-Kurihara; Ahmed Ali; Mika Hiramoto; Yukio Kurihara; Yasmine Abouleila; Eman Muhammad Abdelazem; Takayuki Kawai; Yuko Makita; Mika Kawashima; Tsuyoshi Esaki; Hiroaki Shimada; Tetsuya Mori; Masami Yokota Hirai; Takumi Higaki; Seiichiro Hasezawa; Yoshihiro Shimizu; Tsutomu Masujima; Minami Matsui

doi:10.1093/plphys/kiac093

Tracking metabolites at single-cell resolution reveals metabolic dynamics during plant mitosis

Plant Physiol. 2022 Jun 1;189(2):459-464. doi: 10.1093/plphys/kiac093.

Authors

Emiko Okubo-Kurihara¹, Ahmed Ali^{2

3}, Mika Hiramoto^{1

4}, Yukio Kurihara¹, Yasmine Abouleila^{2

3}, Eman Muhammad Abdelazem², Takayuki Kawai^{3

5}, Yuko Makita¹, Mika Kawashima¹, Tsuyoshi Esaki⁶, Hiroaki Shimada⁴, Tetsuya Mori¹, Masami Yokota Hirai¹, Takumi Higaki⁷, Seiichiro Hasezawa⁸, Yoshihiro Shimizu³, Tsutomu Masujima³, Minami Matsui¹

Affiliations

¹ RIKEN Center for Sustainable Resource Science, Kanagawa, 230-0045, Japan.
² Division of Biopharmaceutics, LACDR, Leiden University, Leiden, The Netherlands.
³ RIKEN Center for Biosystems Dynamics Research, Osaka, 565-0874, Japan.
⁴ Faculty of Industrial Science and Technology, Tokyo University of Science, Tokyo, 125-8585, Japan.
⁵ Graduate School of Science, Kyushu University, Fukuoka, 819-0395, Japan.
⁶ The Center for Data Science Education and Research, Shiga University, Shiga, 522-0069, Japan.
⁷ Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555, Japan.
⁸ Graduate School of Science and Engineering, Hosei University, Tokyo, 184-8584, Japan.

Abstract

Analyzing only one cell allows the changes and characteristics of intracellular metabolites during the chromosome segregation process to be precisely captured and mitotic sub-phases to be dissected at the metabolite level.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Segregation*
Mitosis*