Generation of integration-free induced pluripotent stem cell line (KSCBi012-A) from urinary epithelial cells of a healthy male individual

Young Sam Im; Dae Hoon Yoo; Hyung-Eun Kim; Ji Young Oh; Yong-Ou Kim

doi:10.1016/j.scr.2022.102841

Generation of integration-free induced pluripotent stem cell line (KSCBi012-A) from urinary epithelial cells of a healthy male individual

Stem Cell Res. 2022 Aug:63:102841. doi: 10.1016/j.scr.2022.102841. Epub 2022 Jun 9.

Authors

Young Sam Im¹, Dae Hoon Yoo¹, Hyung-Eun Kim¹, Ji Young Oh¹, Yong-Ou Kim²

Affiliations

¹ Division of Intractable Diseases Research, Korea National Institute of Health, Osong, Cheongju, Republic of Korea.
² Division of Intractable Diseases Research, Korea National Institute of Health, Osong, Cheongju, Republic of Korea. Electronic address: [email protected].

PMID: 35700632
DOI: 10.1016/j.scr.2022.102841

Abstract

A human induced pluripotent cell (hiPSC) line, KSCBi012-A, was generated from a 40-year-old male individual using non-integrating episomal vectors expressing reprogramming factors. The generated hiPSCs were integration-free, expressed pluripotency markers, exhibited the potential for differentiation into three germ layers in vivo, and maintained the normal karyotype. This cell line can be used as a control for a disease model and is available from Korea National Stem Cell Bank.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Epithelial Cells
Humans
Induced Pluripotent Stem Cells* / metabolism
Male
Plasmids