RNA Sequencing Analysis of Saliva exRNA

Methods Mol Biol. 2023:2588:3-11. doi: 10.1007/978-1-0716-2780-8_1.

Abstract

Next-generation sequencing (NGS) methodologies are rapidly developing. However, RNA Sequencing of saliva is challenging due to low abundance and integrity of extracellular RNA, as well as large amounts of bacterial RNAs that may be encountered in saliva. In addition, the literature about human salivary extracellular RNA is very scarce. Therefore, in our chapter, we present the most appropriate protocols for saliva collection, pre- and post-processing, including bioinformatic analysis of salivary RNA Sequencing data. However, the choice of the proper method for RNA extraction, cDNA library preparation, and computational pipeline can make a significant impact on the final quality of data and their interpretation.

Keywords: Bioinformatic analysis; RNA Sequencing; Saliva; Salivary RNAs; Transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Gene Library
  • High-Throughput Nucleotide Sequencing*
  • Humans
  • RNA, Bacterial / genetics
  • Saliva*
  • Sequence Analysis, RNA

Substances

  • RNA, Bacterial